الفهرس 
I. IntroductionOnly 14 pages are availabe for public view
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Abstract
Preeclampsia (PE), a human-pregnancy-specific disease defined as the occurrence of hypertension and significant proteinuria in a previously healthy woman on or after the 20th week of gestation, occurs in about 2–8% of pregnancies. Despite its prevalence and severity, the pathophysiology of PE is still not completely understood. PE is now thought to result from a combination of immunologic, inflammatory, dietary, and genetic factors that lead to the failure of normal trophoblastic invasion and remodeling of the uterine spiral arteries attention in the past decade.
Vascular endothelial growth factor (VEGF), a member of VEGF family, is a major angiogenic factor and potential regulator of endothelial cell proliferation. During pregnancy, VEGF is essential for the proliferation of trophoblasts, the development of embryonic vasculature, and the growth of maternal and fetal blood cells in utero. Several genetic studies on the VEGF gene have identified a number of single nucleotide polymorphism (SNP) which have been performed to elucidate its significance in PE. Emerging evidence suggested that VEGF is elevated in PE and correlates with the severity of disease. This study aims to estimate the association between Vascular Endothelial Growth Factor A (VEGFA) gene single nucleotide polymorphism (rs1570360) and the development of PE in the Egyptian population.
The study populace consisted of 200 pregnant women who were divided equally into two groups:
group (1): 100 of patients with preeclampsia.
group (2): 100 of preeclampsia-free patients.
Collection of blood samples from patients, complete clinical evaluation and laboratory investigations were carried out (Complete Blood Picture, BP, Liver enzymes, Creatinine, Protein in urine, Gravidity, Diagnosis of preeclampsia and it’s complications), DNA extraction and purification, Amplification-refractory mutation system-polymerase chain reaction (ARMS-PCR) and conformation by PCR-Restriction Fragment Length Polymorphism (RFLP-PCR) methods were carried out to investigate the (rs1570360) polymorphisms in VEGFA gene.
The results suggested that VEGFA gene - rs1570360 or (–1154G/A) polymorphism had no association with PE risk in all examined patients. In order to warrant and confirm the associations between VEGFA gene polymorphisms and PE risk, further studies are therefore required. However, AA genotype was found in each severe case associated with serious complications of PE disorder VIII. Conclusion and recommendation
The results revealed that VEGFA gene - rs1570360 or (–1154G/A) polymorphism had no association with PE hazard in all examined patients, results revealed that the rs1570360 (–1154G/A) polymorphisms had no association with PE risk in all examined patients. However, there was a relation between genotyping and the severity of preeclampsia. A strong association was found between AA and severe cases even with the development of complications in these cases.
In order to warrant and confirm the associations between VEGFA gene polymorphisms and PE risk, further studies are therefore required.