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Abstract Pseudomonas aeruginosa is an important opportunistic pathogen in nosocomial infections and responsible for high mortality rates. The main resistance characters in Ps. aeruginosa are the presence of efflux pumps, reduced permeability of the outer membrane and secretion of the β-lactamases. Metallo beta-lactamases are considered of the most important enzymes secreted by Pseudomonas, they are encoded by many genes which could be carried on plasmids or chromosomes. Multiplex PCR is considered an essential application that can be used for locating specific sets of genes by aid of primers. Regarding the high mortality rates and prolonged hospitalization associated with the resistance to antimicrobials, early detection of these resistant strains and its phenotypic and genotypic confirmation is necessary to decide the suitable therapy. The aim of this study was to screen beta lactamase production and its genes such as bla-VIM in Ps. aeruginosa strains isolated from hospitalized patients in Tanta University Hospitals. |