الفهرس 
List of contentsOnly 14 pages are availabe for public view
 |  | from | 139 |  |  |
| | | from | 139 | | |
Abstract
This study aimed to investigate the genotoxic effect of drainage water from different resources on root meristems cells of onion (Allium cepa L). The impact of drainage water pollution on the genetic material of bioindicator was onion (Allium cepa L). was assessed by cytogenetic analysis. Allium cepa L. was germinated in four samples of drainage water in addition to the Nile River water as a control. Wastewater used in this study including agriculture drainage water and liquid industrial wastes resulted from chemical fertilizer industry. Inter simple sequence repeats (ISSR) or microsatellite markers were applied to assess genetic diversity induced by drainage water and genetic relationship among five water resources which reflected water quality. Genomic DNA was extracted from fresh onion roots treated with drainage water from the five different resources. six ISSR primers were initially used for screening water quality. An ISSR fingerprinting technique was used to detect DNA damage in onion treated with drainage water. Polymorphisms became evident through the presence and / or absence of DNA fragments in treated roots compared with the control treated with Nile water. The results obtained from theis study were summarized as followes :- 1- Expousure of onion roots to the waste water showed chromosomal abnormalities strong pollution – dependent. 2- Drainage water induced variety of chromosomal abnormalities which gradually increased from Kfr El–Sheikh drainage water, Menyet El–Nasr.