الفهرس 
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Abstract
Background: The field of laser based photochemical reactions receives a great promising for additional applications especially for targeting cells, pathogens, or molecules. Limited studies have investigated the impact of light-emitting diode on stem cells behavior. Aim: Thus the aim of the present study was to assess the effect of Diode laser irradiations on the proliferation of stem cells isolated from the human dental pulp. Methods: Isolation procedures were according to previously developed protocols for dental pulp stem cells. Human dental pulp tissues were obtained from extracted premolar indicated for orthodontic treatment. Irradiation of the sample groups by LLLT was applied in two doses into 96 wells plates by the diode laser device (970 nm) through the fiber optic (Siro Laser fibers 320) at a distance from the opening of the wells to be accurate for equal distribution of the laser irradiation. To assess the proliferation capacity of the isolated stem cells, MTT assay was performed. Results: There is significant difference between the 3 groups after 24 hours only and no significance after 48 and 72 hours. While after 48 hours, the optical density of DPSCs subjected to group II was found to be significantly higher than those subjected to group III. There was a significant decrease in cell number in both groups by 72 hours. There is a significant difference in the number of stem cells which increased by time in group I. High significant difference in the number of stem cells found for group II which measured after 24 hours-48 hours.