الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 150 |  |  |
| | | from | 150 | | |
Abstract
Colistin is one of the last-line choice antibioticsaccessible for management of infections that resulted from multi antibiotic-resistant organisms, such as Pseudomonas aeruginosa and Enterobacteriaceae. The abuse of colistin antibiotic in humans and animals is coupled with colistin resistance .Colistin has been reused for critical infection especially after distribution of multi antibiotic resistant organisms. Colistin resistant E.coli species has an ability to accumulate resistance genes through horizontal gene transferand this cross-transfer occurs between human and animal products due to using of colistin in veterinary medicine.The mechanism of colistin resistant in E.coli is frequently due to mutational alteration within chromosomal genes encoding biosynthesis of LPS and the 1st plasmid –mediated gene for colistin resistance (mcr-1) was documented in China, Aim of the work The aim of this study was detection of the occurrence of mcr-1 gene in isolated E. coli bacteria obtained from hospitalized patients in Mansoura University Hospital . Conclusion In conclusion, plasmid-mediated colistin resistance gene (mcr-1) was found in 8 (16.0%) cases of 50 isolates of hospital acquired Co R EC so the misuse of colistin in agriculture and the poultry industry may be the main cause of the high incidence of mcr-1 in bacteria from animals and animal products. This issue should be addressed by all appropriate authorities by prohibition the careless use of colistin in agriculture.