الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths world-wide. Recently, MACF1 gene has emerged as a potential therapeutic target in cancer as it is involved in many processes critical for tumor cell proliferation, invasion and metastasis. MiR-34a is a well-known tumor suppressor miRNA. miR-34a could be a negative regulator of Wnt signaling pathway by regulating multiple pathway-associated genes. In order to study miR-34a in colorectal cancer we collected 40 colonic tissues from CRC patients (20) and control subjects (20). miR-34a-5p and MACF1 expression were assessed by real time PCR in all study groups. The results showed highly significant decrease (P<0.01) in miR-34a relative expression in the CRC group (median RQ 0.13) when compared to the benign group (median RQ 5.3) and the healthy control group (median RQ 19.63). MACF1 expression showed highly significant overexpression (P<0.001) in the CRC group (median RQ 33.6) when compared to the benign group (median RQ 7.1) and the healthy control group (median RQ 2.7). miR-34a mimic and inhibitor were transfected in CaCo-2 cell line and the proliferation was assessed. The transfection of the cell line with miR-34a mimic decreased cell proliferation. A significant decrease in MACF1 expression was detected by qPCR after transfection of the CaCo-2 cell line with miR-34a-5p mimic. Our study suggests that miR-34a-5p targets MACF1 gene as a part of the wnt signaling pathway leading to the involvement in the molecular mechanisms of CRC development and progression.