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العنوان
Characterization of natural bioactive compounds produced by isolated bacteria from different compost samples produced from aromatic plants /
المؤلف
El‐Helw, Nourhan Osama.
هيئة الاعداد
باحث / نورهان اسامة الحلو
مشرف / خالد على النسر
مشرف / احمد اسامة الجندى
مشرف / ايمان احمد الجبالى
الموضوع
Functional foods. Fermented foods Health aspects.
تاريخ النشر
2018.
عدد الصفحات
97 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Biotechnology
الناشر
تاريخ الإجازة
14/8/2018
مكان الإجازة
جامعة بني سويف - كلية الدراسات العليا للعلوم المتقدمة - التكنولوجيا الحيوية وعلوم الحياة
الفهرس
Only 14 pages are availabe for public view

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from 124

Abstract

This study aimed to highlight the importance of compost from aromatic plants as a stunning source for several bio active compounds generated from their inhabited thermophilic bacteria. Some of the isolated compounds could have a potential role in the treatment of microbial infections.
A total of forty different thermophilic bacteria were isolated from compost. These isolates were tested for their antimicrobial capabilities against different Gram-positive and negative bacteria using agar diffusion and double layer agar methods. The potential isolates were further identified based on morphological, biochemical and 16S rRNA gene sequencing methods.
They were subjected to submerged state fermentation and the total crude metabolites were recovered using ethyl acetate (EtOAc) extraction. All bioactive metabolites were identified using liquid chromatography coupled with high resolution mass spectrometry (LC-HRMS).
It was observed that two out of forty isolates were active against Gram positive bacteria. These isolates were genetically identified as Bacillus species and their different active metabolites were characterized in the EtOAc extracts using LC-HRMS.
LC-HRMS analysis of EtOAc extracts revealed the presence of active metabolites that are responsible for antimicrobial activities.
To the best of our knowledge, this is the first time to find bioactive antimicrobial metabolites from retrieved compost microorganisms in Egypt. So, compost could be a beneficial area for research as a reliable and continuous natural source for different uncountable communities of bacteria.