الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Seafood benefits to human health are noted for multiple bodily organs and physiological functions. Seafood compares favorably with other protein sources as it offers superior macronutrients in the ideal form of lean proteins combined with omega-3, healthy type of fat, and a wide array of highly bioavailable micronutrients. According to the U.S. Food and Drug Administration (FDA), certain varieties of seafood are excellent sources of potassium, vitamin A, vitamin E and vitamin C, vitamin D, calcium. Eating seafood regularly can also potentially lower your risk for developing colon, breast or prostate cancer. Pregnant women can also benefit from the high levels of protein, zinc and iron found in seafood.
The safety of seafood is increasingly becoming an important public health issue as it an important vehicle for pathogenic microorganisms to humans. This is due to the global rise in the number of people vulnerable to disease coupled with the customary tendency of the world population to consume raw or improperly cooked seafood. Vibriosis has been an important cause of fish production loss and human health risk.
The objective of this study is to detect the prevalence and quantification of Vibrio parahaemolyticus (VP) in 100 (25/ species) of seafood samples; collected from coastal regions and fish markets in Damietta; by traditional bacteriological isolation methods and quantitative real time polymerase chain reaction technique (QRT-PCR) as a molecular method for rapid and sensitive detection. It was found that by using traditional bacteriological isolation methods; the incidence of Vibrio parahaemolyticus were 7 (28%), 1 (4%), 7 (28%), 6 (24%) in examined Shrimp , Crabs , Bivalve molluscs and brush tooth Lizard fish samples, respectively. While molecular detection system(QRT-PCR) revealed that the incidence of Vibrio parahaemolyticus were 20 (80%), 18(72%), 21(84%), 21(84%) in examined Shrimp, Crabs, Bivalve molluscs and brush tooth Lizard fish samples, respectively. In conclusion; these data indicate that QRT-PCR can provide sensitive species-specific detection and quantification of V. parahaemolyticus in seafood without prior isolation and characterization of the bacteria by traditional microbiological methods.
Using a biotechnology application, “ Genome Sequence” a partial genetic analysis was performed for 4 representative samples of each of the four marine fish species studied from the province. The samples were found to be identical to the Vibrio parahaemolyticus strain in the gene bank by (96.7% -99.6%) and to Vibrio Spp. strains by100%.
The application of nanobiotechnology to increase the sensitivity of the Real Time Polymerase Chain Reaction (QRT-PCR) result in reaction sensitivity increase by 3x103 fold.