الفهرس 
Aim of the workOnly 14 pages are availabe for public view
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Abstract
Acute myeloblastic leukemia (AML) is the second most common
hematological tumor and is characterized by increased proliferation
and impaired maturation of early myeloid cells, leading to an
accumulation of immature blood cell.
The HOXA5, HOXA9 and PRDM16 genes are associated not only
with the development of hematologic malignancies but also with the
prognosis of these conditions.
The present study aim to assess the clinical significance and the
prognostic value of quantitative PCR expression of HOXA5, HOXA9
and PRDM16 in newly diagnosed AML patients. The present study
was carried out on 60 newly diagnosed patients with AML and 40 age
and sex matched healthy subjects as a control group, all of them were
subjected to complete history taking, thorough clinical examination,
and laboratory investigations including CBC, BM aspiration with
examination of leishman- stained peripheral blood and bone marrow
smears, and finally detection of the levels of HOXA5, HOXA9, and
PRDM16 expression by Quantitative real time -PCR (Qr-PCR).
In the present study HOXA5, HOXA9, and PRDM16 genes were
highly expressed in AML patients compared to control group. A
significant relation was found between HOXA5, HOXA9, and
PRDM16 and clinical signs in AML patients the highest one was
splenomegaly. The present study reported a significant negative
correlation between HOXA5, HOXA9, and PRDM16 expression and
HB level in AML patients and a significant negative correlation
between HOXA5 and PRDM16 expression and PLT count, while no
significant correlation was found between HOXA9 and PLT count.
Summary and Conclusion
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also there was no significant correlation between HOXA5, HOXA9,
and PRDM16 expression and TLC and BM blasts.
The present study reported that high HOXA5, HOXA9, and PRDM16
expression related significantly with unfavorable cytogenetic.
Moreover AML patients with high HOXA5, HOXA9 and PRDM16
expression had lower rates of complete remission achievement, those
patients also had bad clinical outcome, shorter disease-free survival
and overall survival
from the results of the present study it was revealed that the HOXA5,
HOXA9, and PRDM16 expression can be used as a poor prognostic
predictors for AML. Furthermore, the present study showed that
expression of HOXA5, HOXA9, and PRDM16 were closely
correlated with each other, this correlation is a particularly interesting
finding that call for further research to reveal the underlying
mechanism of interaction.