الفهرس | Only 14 pages are availabe for public view |
Abstract Every disaster is unique and involves interplay of different factors and circumstances such as nature of disaster, number of victims and extent of body fragmentation that eventually challenges the disaster response planning. These events need special planning and preparation designed specifically for situations with potentially thousands of deceased victims. Developments in molecular genetics have allowed studying the person-to-person differences in parts of DNA that are not involved in coding for proteins, and it is primarily these differences that are used in forensic applications of DNA typing for personal identification. Sample preference for DNA analysis can help in proper management of the disaster. Since a disaster is a chaotic environment that can complicate definite identification of the remains. With some planning, and proper sample selection we can reduce stress for those involved in the identification procedure thus increasing the probability that all recovered samples are identified. The goal of this work is to decide which human tissue is preferred over the other for DNA tests for human identification as regards the available samples recovered from different disasters including environmental disasters, fires and explosions. Thus we can ensure precise and quick identification of victims saving time resources and reduce stress of relatives by delivering results accurately and quickly as possible. Furthermore reducing the hazards of failure in victim identification. This cross sectional laboratory based study was conducted at the Egyptian Forensic Medicine Authority (EFMA) at Sayda Zaynab. From May 2016 to April 2017 on 246 samples and tested using manual Qiagen extraction kit, Prep Filer extraction kit or Qiagen automated kit using EZ1. Extracted DNA was then quantified using Quatifiler® Human DNA Quantification kit by Step one Real Time PCR. Samples were amplified using (AmpFlSTR ® Identifiler ® Plus) PCR Amplification Kit (Life Technologies Applied Biosystems) and AmpFlSTR ®Y Filer Amplification Kit (Life Technologies Applied Biosystems). Amplified PCR products were run electrophorethically on a 3130xl or 3500 Genetic Analyzer (Life Technologies Applied Biosystems) and finally analyzed. |