الفهرس 
Alpha Lipoic AcidOnly 14 pages are availabe for public view
 |  | from | 109 |  |  |
| | | from | 109 | | |
Abstract
This study was carried out at a private poultry farm under supervision of Animal Production Department, Faculty of Agriculture, Tanta University, during the period from April to June 2017, to investigate the effect of alpha lipoic acid supplementation levels on broilers performance, immunity, antioxidants status, hematology, some blood and biochemical constituents. Three hundred and sixty one-day-old unsexed Ross broilers chicks were used in this experiment. Birds were individually weighed to the nearest gram, and randomly distributed into four equal experimental groups; with three replicates of 30 chicks each. The basal diet was a commercial cornsoybean meal diet formulated to meet or exceed the nutritional requirement of growing chicks as recommended by the NRC (1994). Dietary treatments evaluated included: 1) Basal diet (control) without any addition 2) Basal diet supplemented with 100 mg ALA/kg diet 3) Basal diet supplemented with 200 mg ALA/kg diet 4) Basal diet supplemented with 300 mg ALA/kg diet Measurements: 1- Performance Trails Body weight and weight gain, feed consumption, feed conversion ratio, mortality rate, slaughter test. Summary 86 2- Biochemical Analysis of Blood Liver function determinations (total protein, albumin, globulin, AST, ALT, triglyceride, total cholesterol, HDL, LDL and VLDL), Kidney function determinations (Serum uric acid) and glucose. Also, Total antioxidants capacity (TAC), Activity of GSH-Px, SOD and MDA were determined 3- Hematological Parameters Hematological examination includes WBCs, RBCs, HGB, HCT, MCV, MCH and MCHC. Obtained results could be summarized as follows: 1- At the end of the experimental period (fifth week of age), body weight of broilers supplemented with 100 mg (ALA)/ kg diet significantly (P<0.01) improved by 6.57% compared to control. While, broilers received 300 mg (ALA)/kg diet significantly (P<0.01) had the lowest body weight followed by those received 200 mg (ALA)/ kg diet by 7.38 and 7.29% respectively, compared to control. 2- Broilers fed diet supplemented with 100 mg (ALA) /kg diet had significantly (P<0.01) the highest body weight and weight gain followed by birds received control diet. The worst values were obtained for those fed 300 mg (ALA)/kg diet. 3- Feed consumption was statistically affected by the treatments during the completely experimental period except at the second week of experimental period. Throughout the completely experimental period, the feed consumption of birds fed diet supplemented with different levels of ALA diet significantly (P≤0.05 or 0.01) decreased with the increasing of ALA level in the diet. 4- The total feed consumption during completely experimental period was significantly (P≤0.01) decreased for broilers fed diet supplemented with 200 mg ALA/kg diet by 3.16% compared to control. At the same time, there was no difference between other treatments and control group. 5- As a result, for the reduction in feed intake and weight gain, feed conversion ratio among treatments was significantly (P≤0.01) impairments by increasing ALA level in the experimental diet. However, at the 3ed, 4th week and whole experimental period the feed conversion ratio of birds received 100 mg ALA/kg diet significantly (P≤0.01) improved by 20.00, 16.23 and 9.18% respectively, compared to control. 6- Mortality percent of broilers fed diet supplemented with 100 mg ALA/kg diet was decreased by (5%) compared with the control group. While mortality rate of groups received 200 or 300 mg ALA/kg diet were in significant decreased by 1.66 % compared to control. 7- There were no significant differences between all treatments in the relative weights of thymus, spleen, gizzard, heart and lungs. On the other hand, there were significant (P≤0.05 or 0.01) differences between treatments for the relative weights of bursa, liver, kidney, abdominal fat and carcass percentage. 8- Superoxide dismutase (SOD) (u/g) was significantly (P≤0.01) increased in thigh muscle by increasing ALA supplementation levels from 100 to 300 mg ALA/kg diet. Thiobarbituric acid reactive substance (TBARS) (n.mol/g) was significantly (P≤0.01) decreased in thigh muscle by increasing ALA supplementation levels from 100 to 300 mg ALA/kg diet. 9- There were no significant differences between all treatments in the count MCV, MCH and MCHC. On the other hand, there were significant (P≤0.05 or 0.01) differences between treatments for the Count of WBCs, differentiation of WBCs, RBCs, HGB and HCT. 10- Birds fed the ALA-supplemented diet had the highest plasma total antioxidant capacity (TAC), superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) enzyme activities, and the lowest plasma malondialdehyde (MDA) activity. 11- There were no significant differences between all treatments in the concentration of cholesterols, VLDL, total protein, albumin and uric acid. On the other hand, there were significant (P≤0.05) differences between treatments for the concentration of triglycerides, HDL, LDL, globulin, ALT and AST activity. In conclusion, the current study revealed that broilers fed diet supplemented with ALA showed significant improvement effects on performance, biochemical and economic efficiency typical to those reported in the literature. Treatment with 100 mg ALA/kg diet succeeded to influence growth performance indicators, immunological, biochemical characteristics, lipid metabolism.