الفهرس 
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Abstract
In this study, plant samples of Withania somnifera, Catharanthus roseus, cupressus sp were collected from different places in Tanta and Shebein EL-kom university camps plant. In order to investigate the ability of endophytic fungal communities to produce Taxol in vitro under optimized conditions.
The present study was applied to test the isolated endophytic fungal strains for their ability to produce Taxol. then quantification of the Taxol content in fermentation medium for the selected producing strain. where the highest content of Taxol recorded was from Cladosporium cladosporioids isolated from Catharanthus roseus.
In order to determine whether the isolated strain is a Taxol producer or not, the culture filtrate was examined by UV absorption at 273 nm, HPLC, TLC and mass spectroscopy that confirms Taxol presence in fermentation medium .
Also investigating biological activity of produced Taxol was tested against human cancer cell lines Hep-G2, human prostate cancer cell line PC-3 and mylogenous carcinoma cell line M-NFS-60 in mice.
Investigating physical conditions ( temperature and pH ) were carried out for determining the optimum conditions for Taxol production by Cladosporium cladosporioids which revealed that optimum incubation temperature for Taxol production is at 25 ̊ C, also the optimum initial pH of the fermentation medium that causes C.cladosporioids to yield maximum amount of Taxol is at pH 5.5.
Several trials to improve Taxol production was applied using some chemical compounds previously known as activators for microbial metabolism including serinol, p-hydroxybenzoic acid, salicylic acid, vanadyl sulphate and benzoic acid which was added to fermentation medium in different concentrations implying the optimum concentration of the chemical activator corresponding to the highest Taxol content extracted from fermentation medium.