الفهرس 
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Abstract
Dental caries is the most common oral disease worldwide. Dental
caries is a complex interaction over time between acid-producing
bacteria, fermentable carbohydrate, and many other host factors including
teeth and saliva.
Theoretically, the inhibition of each step during caries formation
results in the prevention of dental caries. The main goal is to reduce the
microbial load in the mouth by both mechanical and chemical oral
hygiene methods, the effectiveness of mechanical means would depend
on skills and technique of the individual carrying out these procedures,
thus chemical plaque control can be used as an adjunct to mechanical
plaque control procedures.
Chlorhexidine though very effective as an antiplaque agent, also
has certain side effects like brown discoloration of the teeth, oral mucosal
erosion, and bitter taste. So it is important that the dentist must be
familiarized with alternatives to ordinary antibacterial. This i nitiated us to
carry out this study.
This study was designed to evaluate the inhibitory effect of two
different milk proteins Alpha lactalbumin (α-LA) and Casein
glycomacropeptide (GMP) on Mutans streptococci count in dental plaque
with different concentration (1% and 2% of each).
Twenty children patient attending the outpatient’s clinic,
Pedodontics department, Faculty of Dentistry, Suez Canal University,
Ismailia, Egypt, were asked to rinse first with water, then 6 dental plaque
samples were taken from the fully erupted buccal surface of
Summary
83
1
st
permanent molars from each child , by sterile dental excavator and
stored in Epindorff tube, then the plaque samples were transported in
icebox at 4°C to the laboratory of Department of Food S cience and
Technology, Faculty of Agriculture, Ain-Shams University.
Total 120 plaque samples were collected from the patients and
divided into 6 groups equally, 20 per each group, which were divided as
follows
1. Control group
2. Chlorhexidine group (positive control)
3. Alpha lactalbumin1 % (T1)
4. Alpha lactalbumin 2% (T2)
5. Casienglycomacropeptide1% (T3)
6. Casienglycomacropeptide2% (T4)
Then all Epindorff tubes were incubated at 37C° for 1 hour to be disperse
for 30 seconds by vortex mix, then tenfold dilutions were made by
normal saline to have a full colony counting for the caries related
microorganisms.
Mutans Streptococci in plaque of tested children were evaluated using
selective media for Mutans Streptococci (Mitis Salivarius agar).
All colonies with morphological characteristics for Mutans
Streptococci were counted. The countable plates were divided into 4
quadrants; the count of colonies was performed by using the counting
technique in each quadrant separately then summation of the number of
colonies in the 4 quadrants. The results were expressed as (CFU/ml).
Summary
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The results of present study revealed the following:
Chlorhexidine is more potent than Alpha lactalbumin and Casein
glycomacropeptide.
Alpha lactalbumin 2% was more effective in inhibition of Mutans
streptococci than Alpha lactalbumin 1%.
Casein glycomacropeptide 2% was also more efficient in reduction
of Mutans streptococci count than both Alpha lactalbumin 1% and
2%.
Casein glycomacropeptide 2% had reduced the Mutans
streptococci count more than Casein glycomacropeptide 1%.
Casein glycomacropeptide 2% was the strongest intervention in
reducing the Mutans streptococci count.
Alpha lactalbumin 1% was the weakest intervention in reducing the
Mutans streptococci count.