الفهرس 
AIM OF PRESENT WORKOnly 14 pages are availabe for public view
 |  | from | 243 |  |  |
| | | from | 243 | | |
Abstract
Two highly sensitive and selective spectrophotometric and spectrofluorimetric methods have been developed for the determination of triamterene and amiloride hydrochlorid in pharmaceutical dosage forms and/or biological fluids. In this thesis, method (A) are based on the reacts of the studied drugs with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in the presence of borate buffer and method (B) are based on the direct estimation (non- derivatization) of the native fluorescence of the studied drugs. All variables affecting the absorption and fluorescence intensity were carefully studied, optimized and incorporated into respective recommended procedures. Calibration graphs were linear with good detection limits and quantification limits for the studied drugs. A proposed derivatization reaction pathway is presented. The methods were successfully applied to the analysis of commercial tablets and/or biological fluids and the results were in excellent agreements with those obtained using the reference/pharmacopoeial method. The recoveries were 97.7 − 103.2% with correlation coefficients of > 0.999 and relative standard deviations of < 4.3. Statistical analysis of the results revealed the high accuracy and precision of the proposed methods.