الفهرس 
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Abstract
In this study, a total of 200 samples were collected from chicken fecal samples (100) and human fecal samples (100) during 2018 and examined for detection of Campylobacter spp. Out of 100 chicken tested samples, 69 samples were positive for Campylobacter by (PFM), 33 by selective (SEM) and 80 by PCR. While out of 100 human tested samples 23, 55 and 63 were positive for Campylobacter by PFM, SEM and PCR. Molecular detection of mapA gene and ceuE gene for identification of C. jejuni and C. coli, respectively. Results revealed that the percentages of C. jejuni and C. coli detected in chicken fecal samples were 14 % and 86%, respectively. While the percentages from human fecal samples were 34% and 66% respectively. Molecular characterization of Campylobacter 15 virulence genes in a 20 representative positive samples, Results revealed that the prevalence of different tested virulence genes in both chicken and human tested samples could be categorized into 4 different groups as follow: group no. 1: genes detected in all chicken and human samples (virB11, iam, racR and tetO). group no. 2: genes were detected in chicken and human tested samples with different ratios (dnaJ, flaA and cdtB). group no. 3: genes detected in chicken Samples and not detected in human samples (pldA, dnaJ and wlan). group no. 4: genes were not detected in any of tested samples neither chicken nor human (Cst11, csrA, Waac, ggt and cgtB). Gene expression of 23srRNA (housekeeping gene) plus 7 C. jejuni and coli virulence genes from chicken versus human group (control). Data explained that there were significant differences in the expression of 4 out of 7 tested virulence genes of both C. jejuni and C. coli in chicken group vs human group (control). The mean fold change values of the four significant genes were: dnaJ (11.5), virB11 (7.16), flaA (5.39) & iam (3.72) for C. jejuni and dnaJ (8.34), virB11 (5.21), flaA (2.84) & iam (2.5) for C. coli.