الفهرس 
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Abstract
Hirschsprung disease (HD) is diagnosed by absence of ganglion
cells in rectal biopsy. In some cases, standard methods fail to diagnose
agangliosis. The aim of this study is to assess the diagnostic value of
immunohistochemistry (IHC) of calretinin and synaptophysin compared
to standard methods.
This prospective cross section study was conducted in Menoufia
University hospitals, Egypt spanning the period between October 2017 to
December 2018. Rectal biopsies of the clinically suspected HD patients
stained with calretinin and synaptophysin and their results compared to
the standard hematoxylin and eosin (H&E) stained sections.
A total of 30 patients aged from 3 days to 2 years with a male to
female ratio 11:4 were examined for rectal biopsies. Sections of 9 cases
were diagnosed
HD. In inadequate specimens, sensitivity and specificity of
calretinin and synpatophysin (100%, 80%) and (100%, 85.71%)
respectively were superior to the sensitivity (40%) and specificity (14%)
of H&E. However, in adequate specimens, results of H&E, calretinin and
synaptophysin were the same.
Immunohistochemical expression of calretinin and synaptophysin
were conclusive, diagnostic and superior to the results of H&E stained
section in inadequate. However, in adequate specimens calretinin and
synaptophysin were consistent and confirmatory to the results of H&E
sections.