الفهرس 
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Abstract
Acne vulgaris is one of the common dermatological diseases. Sebaceous hyperplasia, follicular hyperkeratinization, and bacterial hypercolonization, as well as immune reactions and inflammations may lead to acne, which has a quite complex pathogenesis and predisposition. The molecular mechanisms involved in acne vulgaris are believed to be due to the action of increased levels of proinflammatory factors including IL-6 and IL-17. Genetic factors play an important role in the pathogenesis of acne vulgaris, which is supported by several studies that showed increased incidence of acne vulgaris among relatives of affected patients and high rates of concordance for acne vulgaris among monozygotic twins when one is affected.
Interleukin-6 is a proinflammatory chemokine which chemo attracts and mediates proliferation and growth of many immune cells. IL-17 is a multifunctional, proinflammatory cytokine. It has been implicated as a key cytokine in the pathogenesis of acne vulgaris because of its ability, alone or in interactions with other mediators, to induce several cytokines and adhesion molecules involved in the development of acne lesions.
It is proven that acne vulgaris had genetic basis as many inflammatory diseases. The clarification of which should permit a better understanding of disease etiology, allowing the improved classification, diagnosis, and treatment.
The present study aimed to evaluate the serum levels of IL-6 and IL-17 and demonstrate genetic polymorphism of IL6- 572 G/A and IL-17 197 G/A in acne patients versus controls. This case-control study was conducted on 40 acne patients and 40 age and sex matched healthy volunteers as a control group. They were collected from the Dermatology and Plastic Surgery Outpatient Clinics, Faculty of Medicine, Menoufia University Hospital during the period from September, 2015 to March, 2016. For all, ELISA and Real Time –PCR were used to evaluate IL-6 and IL-17 serum levels, and to detect genetic polymorphism of IL-6 572 G/C and IL-17 197 G/A genes respectively.
The result of the current study showed that: Interlukin-6 serum levels showed statistically significant higher mean values in acne vulgaris patients than the control group (1.68±1.93 pg/ml vs. 0.65±0.48 pg/ml) (p<0.001). Studying the relation between IL-6 serum levels and studied personal and clinical criteria of acne patients revealed non-significant correlations or associations except for history of previous AV treatment (P=0.036).
Non significant differences were observed between acne vulgaris patients and their controls regarding IL-6 572 G/C genotypes (P=0.258) and allele distribution (P=0.797). Studying the relation between IL-6 572 G/C genotypes and the assessed personal and clinical criteria of acne patients revealed non-significant correlations or associations in acne patients except for age of patients, as G/C genotype was observed in younger cases (P=0.023)
In acne patients, there was a significant increase in IL-17 serum levels in acne vulgaris patients than controls (P <0.001). Studying the relation between IL-17 serum levels and the assessed personal and clinical criteria of acne patients revealed non-significant correlations or associations except for history of previous AV treatment (P=0.027). Compared to IL-17 197 G/A wild type (GG), GA genotype was significantly demonstrated in AV cases (23, 57.5 %) than in controls (8, 20 %) with 10.06 OR, as well AA genotype was observed in cases than controls with 4.88 OR (P<0.001). Additionally, IL-17 A allele was significantly reported in acne vulgaris cases than controls (51% vs. 25%) (P<0.001).
Studying the relation between IL-17 197 G/A genotypes and the assessed personal and clinical criteria of acne patients revealed non-significant associations (p=>0.05 for all).
There was non significant correlation between IL-6 and IL-17 serum levels among acne vulgaris patients(r =-0.236, P =0.143).