الفهرس 
Limbic system
Characterization of ADSCsOnly 14 pages are availabe for public view
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Abstract
Background: Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and other cognitive abilities. Unfortunately, curative measures are not yet available. Adipose-derived stem cells (ADSCs) have a high proliferation capacity in vitro and could differentiate into cells with several neuronal and glial characteristics. Thus, their therapeutic potential could be applicable.
Aim of the work: To evaluate the histological changes of the hippocampus in an Alzheimer’s disease model and to highlight on the possible therapeutic role of ADSCs.
Materials and Methods: Seventy rats were used in this study; of which sixty adult male were used for the experimental groups and ten young rats were used for collection and isolation of ADSCs. The experimental groups were divided into four groups: group I (control group) included 30 rats. group II (Alzheimer’s group) included ten rats which were subjected to induction of Alzheimer’s disease by receiving aluminium chloride orally in a daily dose of 17mg/kg body weight for 75 days. group III (ADSCs-treated group) included ten rats which were subjected to AD induction, then intravenously injected with a single dose of ADSCs (1x106) suspended in 0.5 ml PBS and sacrificed after another four weeks. group IV (Recovery group) included ten rats which were subjected to AD induction and left untreated for four weeks then sacrificed. At the end of the experiment, the Morris water maze test was done for 6 days. Brains were excised and processed for light microscopic and transmission electron microscopic examination. Morphometrical measurements and statistical analysis were performed.
Results: Rats of groups II and IV showed fluctuation in the time required to reach the platform in the Morris water maze test. Loss and degeneration of the pyramidal cells were detected in areas of Cornu Ammon’s (CA1, CA3) of the hippocampus proper and of granule cells in the dentate gyrus (DG). Deposition of amyloid plaques and neurofibrillary tangles were evident. Treatment by ADSCs showed improvement of the histological appearance in all areas examined.
Conclusion: The ADSCs showed a high therapeutic efficiency in treating AD experimental model in rats and could be a challenging therapeutic measure.