الفهرس 
ACKNOWLEDGEMENT
Copper and inflammationOnly 14 pages are availabe for public view
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Abstract
In this submitted work, the immunosuppressor AFB1 was experimentally synthesized from an authentic sample of aspergillus flavus. The toxin was harvested, isolated, purified and collected as a fine powder after getting rid off the organic solvent chloroform. Induction of aflatoxicosis was performed by this isolated AFB1 in a suspension of dairy milk taken orally to exposed experimental white rats for four weeks (chronic intoxication).
Such an exposure successfully induced chronic aflatoxicosis in the tested animals. characterization of WBCs counts (lymphocytes, neutrophils, and monocytes) in the systemic blood showed significant reduction.
The histopathological examination of tissue sections from the aflatoxicosed animals as liver showed hyperplasia of bile duct, presence of numerous fat globules and increase of the collagenous fibrous tissue. Also lymphoid tissues revealed depletion of lymphocytes from spleen and lymph node and wideness of intercellular space.
The considered therapeutic agent copper (II) albumin complex that was synthesized, purified, and prepared for biological use in the same vehicle dairy milk. Treatment by such tested copper complex showed significant reduction in the oxidative stress parameters as well as improvement in the tested WBCs counts and immunoglobulins. The investigated tissue slices by optical and electron microscopy verified cellular mediated immunity that was potentiated after suppression during aflatoxicosis.
The improvement in the measured immunological parameters on the level of cellular mediated features or on the level of immunoglobulins (humoral immunity) as well as tissue synthesized interleukins was collectively, achieved by an speculated an induction of copper signaling on the affected targeted cellular tissues.
Basically, the reviewed work of others engaged in copper signaling for cellular and biochemical changes from prokaryotes up to human cellular tissues promote rational interpretation of our deduced results. Such remarked events may established as possible digested copper complex in ligands protein with circulating albumin in extracellular spaces as well as alpha1 globulin. These copper ligands deliver cu2+ to the target cell membrane to be reduced to cu1+ by specific ferroreductase. The reduced copper be transported by specific chaperon as ligands that potentiate transcription of specific proeins such SOD, catalase, immunoglobulins as well as interleukins that potentiate cellular mediating immunity