الفهرس 
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Abstract
This study was carried out on fertile patients with history of fertility
within 2 years visiting Andrology Outpatient Clinic in Suez Canal
university hospital and a relevant private sector (Cleopatra- IVF center-
Ismailia) in Ismailia-Egypt.
The study is descriptive cross sectional study in which 70 fertile
patients were studied after taking an informed consent aimed at assessing
the percentage of sperm chromatin condensation among normal fertile
males and exploring the correlation between percentage of DNA
fragmentation and seminal parameters in fertile males. History, clinical
examination, semen analysis and sperm chromatin condensation assay
using acidic aniline blue were carried out. The semen parameters were
measured using simple light microscopy according to the World Health
Organization (WHO 2010) criteria.
Total number of patients was 70 fertile males. The mean age of
participants was 32.57±6.86 years (ranged from 23 to 60 years), weight
was 74.40±5.88, height was 173.97±6.03 and BMI was 24.52±1.19. The
majority of patients (45 patients; 64.3%) had normal BMI (< 25.0 kg/m2).
The majority of participants live in urban areas (51 patients (72.9%).
The mean sperm count (concentration) was 73.42±35.39, the mean of
progressive sperm motility (A+B) was 51.14±5.78, the mean of total
sperm motility was 70.92±6.32, the mean of sperm viability was
81.07±6.00 and the mean percentage of normal forms of sperm was
47.02±8.49.
Summary
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Regarding macroscopic characteristics of semen, the color of semen
samples in all populations was gray; and liquefaction time was normal in
all populations, seminal volume was 3.19±0.71; the mean of pH was
7.45±0.12, the viscosity was normal in the majority of patients (69
patients, 98.6%), while viscosity increased in 1 patient (1.4%).
The mean of sperm DFI (abnormal sperm chromatin condensation)
using acidic aniline blue stain was 7.95±2.68. Also, there were negative
correlation between DFI and semen parameters (motility, volume, and
concentration) and there were positive correlation between DFI and each
of age and BMI.