![]() | Only 14 pages are availabe for public view |
Abstract Summary This study aimed to assess seminal Sirt-1 expression in infertile men before and after varicocele repair. Overall, 53 men presented to the University hospitals after ethical approval and informed consent were investigated. They were allocated into: I- Fertile healthy men (n=23) as controls 2-Infertile OAT men associated with varicocele (n=30) Inclusion criteria: Stable relationship, confirmed abnormal semen parameters and normal female partner. Exclusion criteria: Leucocytospermia, hormonal therapy, immunological disorders, dyslipidemia, hypogonadism, cardiovascular disorders, morbid obesity, and hepatic/renal failures. They were subjected to: History taking Clinical examination Semen analysis according to WHO guidelines (2010). Estimation of seminal SIRT-1expression by Western Blot technique. Surgical repair of varicocele followed up for 3 months. The results were as follows: Seminal SIRT-1 showed significant decreases in infertile OAT with varicocele compared with fertile men. Also, seminal SIRT-1 showed significant increases in infertile OAT with varicocele 3 months post-operatively compared with the pre-operative data. Seminal SIRT-1 (ng/ml) showed significant decreases in infertile OAT men with grade III varicocele compared with infertile OAT men with varicocele grade II. Seminal SIRT-1 showed significant increases in infertile OAT men with varicocele post-operatively compared with the pre-operative data Seminal SIRT-1 expression showed significant positive correlation with sperm concentration, sperm motility, sperm normal forms and nonsignificant correlation with the age. Conclusion Seminal Sirt-1expression has a role of male infertility being significantly decreased in infertile OAT men associated with varicocele and significantly increased after varicocele repair. |