الفهرس | Only 14 pages are availabe for public view |
Abstract Toxicity studies ■ Venom manually and electrically extracted before storage The LD50 values of the venoms for the two honeybee subspecies using two extracted methods revealed that, the Egyptian bee venom has significally higher toxicity than Carniolan bee venom. The venom electrically stimulated has significant higher toxicity than the manually extracted. The present results of spectrophotometer and electrophoresis studies were supporting to these results of the toxicity studies. ■ Venoms electrically extracted after storage for 12 and 24 months The toxicity was significantly decreased in the different storage conditions for venoms of the two subspecies. In general, the lowest values of the reduction (%) in the toxicity were under the freezer storage condition while the highest values of the reduction (%) were under storage at room temperature in the light for the two bee venoms during the storage periods. The Egyptian bee venom has significantly higher toxicity under all storage conditions during storage periods. The reduction (%) in the toxicity of the Carniolan bee venom was higher than that in the Egyptian bee venom all storage condition overall the storage periods except under freezer condition was slightly higher in the Egyptian bee venom. These results were also supported by the present results of spectrophotometer measurements of venom samples stored under freezer conditions after 12 and 24 months. Finally, based on the present results of spectrophotometer, electrophoresis and toxicity studies and comparative analysis between Egyptian and Carniolan bee venoms and different storage conditions, this study recommends: • Freezing was the best storage conditions for honeybee venom within the range that assures preserve maximum activity of their components, stability and prolonged storage with high quality and efficiency. • It also recommends that the bees concerned to expand the breeding of the Egyptian honeybee subspecies, maintain it and encourage the beekeeping to commercial production of the Egyptian bee venom due to it has high biological activity and toxicity than that of the Carniolan subspecies and is the better form for potential pharmacological source. |