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العنوان
Advanced studies on some endophytic
microorganisms and plant extracts to
control root-knot nematodes /
المؤلف
Younis, Dalia Mohamed El-Sayed.
هيئة الاعداد
باحث / داليا محمد السيد يونس
مشرف / الشوادفي منصور موسى
مناقش / أحمد محمد جمال
مناقش / مجدى السيد مهدى
الموضوع
Plants - Microbiology. Probiotics.
تاريخ النشر
2018.
عدد الصفحات
255 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علوم النبات
الناشر
تاريخ الإجازة
29/10/2018
مكان الإجازة
جامعة المنوفية - كلية الزراعة - أمراض النبات
الفهرس
Only 14 pages are availabe for public view

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from 255

Abstract

In this study four different plants were used i.e. cabbage, cassia, moringa and chinaberry at different forms i.e. chopped leaves, powder or extracts; different concentrations and depths. The four plants under study used as chopped leaves as soil fumigants. Results revealed that moringa at 4%the most effective one followed by cabbage at 4%. Te lowest effect recorded with cassia 1%.The four plants were used as a chopped leaves at 4% at tree depths 5, 15 and 30 cm against nematodes. Results revealed that the depth of 15 cm the best one in reducing the nematode population. The different four plants used as a leaves powder at 1, 2 and 4% of soil weight. Moringa at 4% the effective one in reducing nematode population, whereas cassia at 1% the lowest one.Using seeds powder of moringa and cabbage at different doses 1, 2 and 4% of soil weight. Moringa seeds powder at 4% the highest effective one on reducing root-knot nematodes. Moringa oil was used to manage root-knot nematodes At different concentrations 0.25, 0.5, 1 and 2% at different application types i.e. soil drench, root soaking for 30 min. and foliar spray. Results showed that soil drench the effective type in managing nematode population at 2%.The four plants were used also with three different solvents i.e. water, ethanol and ethylacetate extracts under laboratory against egg hatching and larvae mortality and under greenhouse conditions. Results showed that moringaethanol extract was the effective one either in vitro or in vivo. Moringa leaves as the effective one was analyzed by HPLC and results showed that the leaves contains some effective phenolic compounds which play a promise role in managing nematodes. Eleven isolates of bacterial and six of fungal isolates were isolated from cabbage and moringa roots and seeds (Endophytic), identified and evaluated against root-knot nematodes in vitro and in vivo. Results found that the all bacterial and fungal isolates appeared effectiveness against egg hatching and larvae mortality in vitro and also on nematode population in vivo.The integrated pest management (IPM) carried out by using the effective treatment from each experiment to manage root-knot nematodes under greenhouse conditions. Results found that all treatments reduced all nematode parameters. Trichoderma plus B. cereus appeared a promising in managing root-knot nematodes.