الفهرس | Only 14 pages are availabe for public view |
Abstract Infectious bronchitis is one of the most common pathogens in the poultry industry worldwide. It adversely affects poultry producers and their industry. It is considered one of the most important diseases affecting the respiratory system and some of the strains have the ability to infect the kidneys in small chickens, leading to inflammation of the kidney, which can cause a high level of mortality among the herds of chickens. This disease is characterized by a lot of varieties in the genetic formation, especially the protein S1, resulting in the emergence of new strains of the disease, which leads to the reduction of immunity caused by vaccination. In this study, the prevalence of IBV was assessed in Egyptian poultry farms, where 200 farms from Sharkia Governorate and 200 farms from other governorates (Fayoum, Ismailia, Beheira and Dakahlia) were examined over the past three years (2014, 2015, 2016) using real time PCR (rRT-PCR) series that found 113 farms were positive with percentage 28%, also the results showed that 78 of the 345 vaccinated herds were found positive (23%), while the non-vaccinated herds (35) of 55 samples showed positive results for the disease. The results of isolation of the virus in SPF eggs showed hemorrhage, dwarfing, and twisting in the embryo injected with the virus. Five passages were performed in the present study before the virus isolation attempt was considered negative. In this study, |