الفهرس | Only 14 pages are availabe for public view |
Abstract Being a major social and medical problem, sexual dysfunction may occur in both sexes. Sildenafil citrate Viagra is selective inhibitor of phosphodiestrase 5 which is present in both vascular tissue and brain, mainly cerebellum. Inhibition of phosphodiestrase 5, causes increased blood flow in the ischemic areas. Cerebellum contributes to coordination of movement and some cognitive functions as attention and language. This work was to clarify the histopathological effects of sildenafil citrate on the cerebellar cortex of adult male albino rats.24 adult male albino rats weighing 200-250 gm were divided into four groups (6 rats were included in each group). The rats of treated groups (II, III and IV) received respectively, 0.25mg/kg, 0.70mg/kg and 1.43mg/kg body weight of sildenafil citrate dissolved in distilled water daily for 30 days. And the first group was the control group received equal volume of distilled water. At the end of experiment, cerebellar cortices were processed for light microscopy, GFAB immunostaining and transmission electron microscopic examination. On examination of low dose treated, cerebellar cortical architecture was preserved, but partially congested and dilated vessels were noted in all layers of cerebellar cortex and also in between folia. In rats treated with 0.7mg/kg, most of purkinge cells appeared irregular, deeply stained with ill-defined nuclei, In high dose treated group, diffuse vacuolations, shrunken deeply stained purkinje cells with pyknotic nuclei were observed. An increase in GFAP immunoreaction was noted in all layers in both intermediate and high doses treated groups. from the present study it was concluded that low dose of sildenafil citrate improved the blood flow with resulting in hyperemia of cerebellar cortex of adult male albino rats. On the contrary high doses of sildenafil citrate resulted in neurodegeneration and reactive astrocytosis mainly in the purkinje cell layer of cerebellar cortex of adult male albino rats . |