الفهرس 
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Abstract
This study carried on 95 pregnant women recruited from antenatal
outpatient clinic and obstetric departments of Shebin El-Kom Teaching
Hospital. The aim of the work was to evaluate the serum leptin levels in
preeclampsia patients and in normotensive pregnant women, as well as, to
assess an association with the severity of the disease.
The study included 95 participant categorized as flow:
-group (A) women with mild preeclampsia (20 cases)
- group (B) women with severe preeclampsia. (45 cases)
- group (C) women with normal pregnancy (30 cases) had been taken as
control group
All patients were subjected to full history, complete general and
obstetric examination was done followed by obstetrical ultrasound. Dipstick urine test were done to detect proteinuria in association with full
laboratory investigations (CBC, SGOT, SGPT, PT, creatinine and uric
acid). A single venous blood sample 5 ml was taken from all groups under
strict aseptic measures, samples was left to stand at room temperature for at
least 30 minutes to allow the blood to clot, then centrifuged for 5 minutes,
frozen at (-20 °C), Each sample was labeled with patient’s name and
identification number and kept without thawing till the day of testing, the
serum leptin was measured using ELISA kits
As regard demographic data, there were no statistically significant
difference regarding maternal age, gestational age and parity.
Serum leptin level were significant statistical higher in preeclamptic than
non-preeclamptic pregnancies, according to the severity, the results showed that maternal serum leptin is significantly higher in severe
preeclampsia than mild preeclampsia. The mean of serum leptin in non
preeclamptic was 13.87 ng/ ml, 30.32 ng/ ml in mild preeclamptic group
and 49.37 ng/ ml in sever preeclamptic group.
Maternal serum leptin levels were found to correlate positively with
systolic, diastolic blood pressure, proteinuria and uric acid.
ROC curve analysis has shown that a cut-off value ≥ 22.1 ng/ml can be
used to detect the presence of preeclampsia with sensitivity of 90.8%,
specificity 92.4%, while cut-off value ≥ 43.0 ng/ml can be used to detect
the severity of preeclampsia with sensitivity of 75.2%, specificity 100%.