الفهرس 
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Abstract
Diagnostic and Biochemical characterization of Hepatitis C Virus Envelope Protein Using Specific Monoclonal Antibodies
Hepatitis C virus was proven to be a major disease over the world until the present and especially in Egypt. The aim of this study was to detect HCV E1/E2 antigens using specific generated mouse monoclonal antibodies as an alternative approach for the confirmation of HCV viremia. Propagation of four hybrid cell lines (7G9(, )6D11(,)3F6( and )6E4) producing mouse monoclonal antibodies, purification and characterization were performed. Peptide mapping for these monoclonal antibodies was done by ELISA as well testing against positive HCV samples and negative samples was carried out. Relying on these tests, the monoclonal antibody (7G9) giving the highest value was selected to fulfill this study. The molecular weight of monoclonal antibody (7G9) was determined by SDS-PAGE and the Western Blot transfer method where E1 was identified at 31 kDa and E2 at 63 kDa. High specificity was shown when testing the monoclonal antibody (7G9) against antigens derived from some bacterial and viral pathogens.The sensitivity of the monoclonal antibody (7G9) was detected till 2.00 µg/ml of the coated antigen HCV E1 (a.a 315-323) . The ELISA and the Dot-ELISA were optimized to detect HCV infected samples where ELISA had a sensitivity of 80.0% detecting samples, a specificity of 96 % and an efficiency of 82.2% and Dot-ELISA had a sensitivity of 76.8% detecting samples, a specificity of 88.0 % and an efficiency of 78.5%. The assays were shown to be reliable in the confirmation of HCV infection However, there was no correlation between serum HCV viral load and HCV E1/E2 antigen detection.
Keywords: Hepatitis C Virus (HCV) - Envelope Protein (E1/E2) - Hybridoma cell line (7G9).