الفهرس 
Introduction and Aim of the WorkOnly 14 pages are availabe for public view
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Abstract
There is a vital need to discover a better treatment for cancer because of its fast spreading rate in the present era. Recently a significant movement has been made in the direction of understanding cancer growth and treatment.
The use of chemical drugs is declining because of its side effects. For these reason, drugs extracted from natural sources are used instead. The bioactive molecules in animal venoms have stimulated new pharmaceutical discoveries. Venoms of several animal species including snakes, scorpions and bees as well as their biochemical derivatives have shown therapeutic potential against cancer as Captopril and Escozul.
The presented study was admitted to compare between the anticancer effects of Naja h., Leurius q. and Apis m. venoms on breast (MCF-7) and colon (Caco-2) cancer cell lines.
This study investigated the following parameters:
The morphological observations and growth rates of MCF-7 and Caco-2 cells before and after treatments were evaluated by inverted microscope.
SRB assay was carried out to evaluate the cytotoxicity of 5-FU and Naja h., Leurius q. and Apis m. venoms on both cell lines.
Semi-quantitative RT-PCR was carried out to analyze the expression profiles of some genes that have a role in programmed cell death (apoptosis).
ELIZA was done to evaluate the oxidant/antioxidant profile was evaluated after IC50% treatments on MCF-7 and Caco-2 cell lines.
The study results can be summarized as follows:
Naja h. venom on MCF-7 cells, the venom stimulated the P53 and BAX genes expression and inhibited the Bcl-2 gene expression. The Naja h. venom was elevated the NO content in MCF-7 cells with depletion in GSH content. Naja h. venom on Caco-2 cells, the venom stimulated the P53 gene expression and inhibited the Bcl-2 gene expression. The Naja h. venom was elevated the NO content in Caco-2 cells with depletion in GSH content.
Leurius q. venom on MCF-7 cells, this venom stimulated the P53 and BAX genes expression and inhibited the Bcl-2 gene expression. The Leurius q. venom was elevated the NO content in MCF-7 cells with depletion in GSH content. Leurius q. venom on Caco-2 cells, the venom stimulated the BAX gene expression and inhibited the Bcl-2 gene expression. The Leurius q. venom was elevated the NO content in Caco-2 cells with increasing in MDA.
Apis m. venom on MCF-7 cells, this venom stimulated the P53 and BAX genes expression and inhibited the Bcl-2 gene expression. The Apis m. venom was elevated the NO content in MCF-7 cells with depletion in GSH content and increasing in MDA. Apis m. venom on Caco-2 cells, the venom stimulated the P53 and BAX genes expression and inhibited the Bcl-2 gene expression. The Apis m. venom was elevated the MDA in Caco-2 cells with depletion in GSH content.
5-FU on MCF-7 cells, 5-FU inhibited the Bcl-2 gene expression. 5-FU elevated the NO content in MCF-7 cells. 5-FU on Caco-2 cells, 5-FU inhibited the Bcl-2 gene expression. 5-FU was elevated the MDA and NO content in Caco-2 cells with depletion in GSH content.
Finally, it can be concluded that snake (Naja h.), scorpion (Leurieus q.) and bee (Apis m.), venoms have anticancer potentials on human breast and colon cancer cells and this is positively related to the antioxidant profile and apoptotic gene expression.
The following recommendations are offered:
• This study recommended that the venoms may insert in cancer treatments.
• This study recommended that the 5-FU is a very toxic compound.
• This study recommended that Naja h. venom may be the best venom to treat MCF-7 cells.
• This study recommended that Apis m. venom may be the best venom to treat Caco-2 cells.
The following points are offered for further research in this field:
• That needs more studies on a comparison between the effect of venoms in vivo and in vitro and by adding S9 faction.
• That needs more studies on a comparison between venoms and chemical drugs and combinations.
• That needs more studies on the venoms compositions and its relation with mode of action.
• That needs more studies on other toxins.
• That needs more studies on other cancer types.