الفهرس 
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Abstract
This thesis represents a pilot study for the investigating the prevalence, identification and antibiogram of Vibrio species in fish and shellfish samples from Port Said coast. This study was carried out during the period from April 2014 to November 2014. A total number of 250 seafood samples, 50 samples each of mullet, sardine, shrimp, cuttlefish and mussel were collected.Overall, 32% of the mullet, 36% of sardine, 52% of shrimp and 44% of both cuttlefish and mussel samples were found to be positive for Vibrio species. Four Vibrio species were identified. V. alginolyticus was found to be the dominant identified Vibrio species with total prevalence of 46.2% (48/104) followed by V. damsela 24% (25/104), V. harveyi 18.3% (19/104) and V.parahaemolyticus 11.5% (12/104). Results of antibiogram showed that antibiotic resistance pattern varied between strains. In general, all isolates showed high sensitivity (100%) to Chloramphenicol and Norfloxacin. Sensitive to moderately sensitive results were recorded for Cefotaxime, Erythromycin, Nalidixic acid and Oxytetracycline. However, 100% resistances of the isolates were observed with Furazolidone with variable percentage resistance (80-100%) to Penicillin G and Ampicillin. The molecular analysis carried out on a selection of 8 isolates, gave positive results using PCR assay.