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Abstract The work presented in this thesis consists of three parts: part I represents a phytochemical investigation of Withania somnifera (L.) Dunal naturally growing in Egypt , part II represents TLC analysis of different organs of the plant that led to the selection of the leaves as the best organ with the highest withanolide content. Part III includes biological evaluation of different plant organs as well as some of the isolated compounds for anti-Alzehimer effects and anticancer effects. Optimization of withanolide extraction process has been studied. Part I : PHYTOCHEMICAL INVESTIGATION This part consists of three chapters: Chapter 1: chromatographic screening of withanolides in different organs: chromatographic screening of different organs and extracts of W. somnifera growing in Egypt was performed using TLC identification method reported in USP35-NF30 monogaraph of Ashwaganda root in order to identify the most promising organ with the highest content of withanolides. from the results obtained, it was concluded that the leaves is the organ of choice for further investigation of withanolides in the plant as it contained the highest amount of withanolides. <Chapter 2: Isolation of nine compounds from the leaves (WSd1- WSd8 and WSbd): TLC screening of different fractions of the dried leaves of W. somnifera using the above reported method revealed that the methylene chloride fraction contains the highest withanolide content in comparison with other fractions. So, it was subjected to further phytochemical investigation leading to the isolation of nine compounds. |