الفهرس 
Acinetobacter Baumannii
Species IdentificationOnly 14 pages are availabe for public view
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Abstract
A. baumannii has emerged as an important and problematic human pathogen as it is the causative agent of several types of healthcare-associated infections including pneumonia, meningitis, wound infection, septicaemia and urinary tract infection especially if these infections associated with medical devices.
As a result of both intrinsic and acquired mechanisms, multidrug-resistant A. baumannii has been reported worldwide and is now recognized as one of the most difficult healthcare-associated infections to control and to treat .MDR A. baumannii is not a new or emerging phenomenon, but A. baumannii has always been an organism inherently resistant to multiple antibiotics .
The potential ability of A. baumannii to form biofilms might also explain its outstanding antibiotic resistance, survival properties and increased virulence. Study of microbial biofilms has received significant attention over the past decades.The present study was chosen to investigate biofilm formation and multiple drug resistance among clinical isolates of A. baumannii and to Verify the existence of any association between biofilm formation and the presence of extended spectrum of β - lactamase (ESBL) blaPER-1 gene in order to understand how this pathogen persists in the hospital environment and causes outbreaks.
This study was conducted on Thirty isolates of A. baumanii collected from different clinical samples namely blood,sputum ,pus from ICU in Ain Shams University Hospitals during the period from June 2015 till Dec 2015. All isolates were identified by conventional microbiological methods and investigated by the following tests; Antimicrobial susceptibility testing using 10 different therapeutically relevant antibiotics by Kirby Bauer disk diffusion method, minimal inhibitory concentrations (MICs) for imipenem with E tests, Biofilm production by using microtitre plate method and PCR for blaPER-1 gene detection.
The results of this study showed that:
- The highest rate of resistance was toward the following antimicrobials; ceftazidime (96.7%), levofloxacin (93.3%) and aztreonam (93.3%)- The least resistance was toward the imipenem (63.4%). - -29 out of 30 isolates (96.7%) were positive for ESBL production.
- 16 out of 30 isolates (53.3%) were biofilm producers; eight isolates (26.7%) produced strong biofilm, 3 isolates (10%) produced moderate biofilm and 5 isolates (16.7%) produced weak biofilm.
- -Resistance to four antibiotics namely amikacin (93.7% vs. 71.4%), levofloxacin (100% vs. 85.7%) , aztreonam (100% vs. 85.7%) and cephepime (87.5% vs. 85.5%) were comparatively higher among biofilm producers than non-biofilm producers.
- -15 out of 30 isolates (50%) had bla PER1 gene.
- There is no significant relation between biofilm formation, MDR and bla PER1 gene among the clinical A.baumannii isolates.