الفهرس 
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Abstract
The aim of this study is to assess the anti-inflammatory potential of
tulathromycin- a macrolide antibiotic- invivo. Nine male rabbits ranging from
2000g to 2500g are used for the experiment. They are randomly assigned to a
group of three: group A: control group that is not given anything and are kept
purely for comparison, group B: vaccine-only group, they are administered a
polyvalent Pasteurella vaccine only, and group C: test group, they are given
tulathromycin 2.5mg/kg body weight and a polyvalent Pasteurella vaccine for
immunostimulation after one half-life of the drug in the rabbit’s body. Blood
samples are drawn and we test several cellular inflammatory biomarkers of
phagocytic activity of neutrophils, macrophage activity, lymphocyte activity, and
assay of DNA damage- COMETassay/SCGE. We also perform histopathological
examination to assess the efficacy of tulathromycin as anti-inflammatory and
immunomodulatory agent in the lung tissues. Blood samples are drawn at zero
time, days 1, 3, and 21 after drug exposure and collected aseptically in heparinized
vaccutainers.
The trial shows statistically significant depression of superoxide anion
secretion by neutrophils and statistically significant decrease in the percentage of
active neutrophils that phagocytosed nitro blue tetrazolium dye ex-vivo 1 and 3
days after injection. Both tests show non-statistically significant change between
test scores on various days, which may connote stabilization of neutrophilic
function in test samples.
The experiment shows a statistically significant decrease in nitric oxide
production by macrophages after invitro cultivation 1, and 3 days after
commencing the experiment. Which somewhat provides certainty in decreased
oxidative stress by macrophages in test samples.