الفهرس | Only 14 pages are availabe for public view |
Abstract The aim of this study is to assess the anti-inflammatory potential of tulathromycin- a macrolide antibiotic- invivo. Nine male rabbits ranging from 2000g to 2500g are used for the experiment. They are randomly assigned to a group of three: group A: control group that is not given anything and are kept purely for comparison, group B: vaccine-only group, they are administered a polyvalent Pasteurella vaccine only, and group C: test group, they are given tulathromycin 2.5mg/kg body weight and a polyvalent Pasteurella vaccine for immunostimulation after one half-life of the drug in the rabbit’s body. Blood samples are drawn and we test several cellular inflammatory biomarkers of phagocytic activity of neutrophils, macrophage activity, lymphocyte activity, and assay of DNA damage- COMETassay/SCGE. We also perform histopathological examination to assess the efficacy of tulathromycin as anti-inflammatory and immunomodulatory agent in the lung tissues. Blood samples are drawn at zero time, days 1, 3, and 21 after drug exposure and collected aseptically in heparinized vaccutainers. The trial shows statistically significant depression of superoxide anion secretion by neutrophils and statistically significant decrease in the percentage of active neutrophils that phagocytosed nitro blue tetrazolium dye ex-vivo 1 and 3 days after injection. Both tests show non-statistically significant change between test scores on various days, which may connote stabilization of neutrophilic function in test samples. The experiment shows a statistically significant decrease in nitric oxide production by macrophages after invitro cultivation 1, and 3 days after commencing the experiment. Which somewhat provides certainty in decreased oxidative stress by macrophages in test samples. |