الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Acute lung injury is a population disorder of acute inflammation that causes disruption of the pulmonary endothelial and epithelial barriers and it is a significant source of morbidity and mortality in critically ill patients. LPS is a major component of the cell wall of Gram negative bacteria (GNB). It plays an important role in the acute lung injury (ALI) pathogenesis. It widely used to induce lung injury in animal models by increasing the intrapulmonary cytokines and the development of typical diffuse alveolar damage.
In the present study, MSCs are clonogenic and self-renewing cells that have capability differentiating into multiple cell lineages within a single germ layer and reconstruction of functional tissues in vivo. They are multipotent cell but in later life adult stem cells are tissue-specific, capable of maintaining, generation, and replacement of aged or damaged cells within an organ.
Firstly, In the present study, MSCs were isolated from mice bone marrow, grown and characterized morphologically by microscopic examination that revealed aggregates of adherent cells which later show plastic adherence on tissue culture flask and homogenous fibroblastoid reaching (70~80%) confluence from (7th till 14th d).
Also, flow cytomety revealed that cells were uniformly negative for hematopoietic cells surface marker CD34, and positive for MSC cell surface marker CD105.