الفهرس 
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Abstract
This study aimed to find whether Osteopontin (OPN) rs9138 and rs1126616 single nucleotide polymorphisms were associated with increased risk of cancer of the colon and rectum. This study included 100 CRC patients and 100 apparently healthy individuals. All subjects were investigated for OPN rs9138 and rs1126616 genotyping and measurement of lipid profile, and tumor markers CEA, CA 19-9 and OPN plasma levels. The genotypes were assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Tumor markers serum levels were measured by enzyme-linked immunosorbent assay. The results revealed that genotype AC of rs9138 and CC and CT of rs1126616 were associated with increased risk of CRC. The C allele of both rs9138, rs1126616, and the haplotypes C (rs1126616)-C (rs9138) & C (rs1126616)-A (rs9138) were associated with increased CRC risk. Serum OPN protein expression in CRC patients was significantly increased as compared to healthy controls.This result concluded that the rs9138 and rs1126616 of OPN were associated with increased CRC risk, and the OPN serum level could be utilized as a possible tumor marker of CRC.