الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
The main objectives of this study were to assess the genetic diversity and phylogenetic relationship among three Egyptian rabbit breeds (Gabali, Baladi Red and Baladi Black) along with New Zealand White breed as a reference breed by using two different molecular markers (RAPD markers and microsatellite markers ). A total of one hundred and twenty animals were used in this study, 30 animals from each breed. The samples were taken randomly from pedigreed animals with the least relationship (avoiding full-sib and half-sibs mating) to decrease the genetic similarity between the genotyped animals. Genomic DNA was extracted from leukocytes and PCR amplification was performed. The amplification products were resolved by electrophoresis; Images were captured and analyzed, then the results were statistically analyzed.
In the present study, RAPD technique was used to assess the genetic variability and phylogenetic relationship among the four rabbit breeds. Fourteen random primers were tested to amplify pooled genomic DNA from these breeds. Ten of them were chosen for further analysis, on the basis of the presence of reproducible and distinct RAPD profiles in one or more rabbit breeds. Out of 131 bands scored using these primers, 74(56.48%) were recognized as Polymorphic and 57 (43.52%) as monomorphic bands. The highest percentage of polymorphic bands was recognized for primers OPB-02 (94%) and OPB-07 (92%). While, the lowest percentage of polymorphic bands was recognized for primers OPA-02(16%) and OPF-12 (28%). The band sharing frequencies (BSF) was found higher between Baladi Red -Baladi Black (0.80± 0.038) , followed by Gabali - Baladi Black (0.71±0.079 ), New Zealand White- Baladi Black (0.70±0.096), New Zealand White- Baladi Red (0.69±0.088) and the least BSF was found between New Zealand White- Gabali (0.64±0.081). Overall, there was no significant difference (P>0.05) in BSF values between breeds. The highest genetic distance was found between Baladi Red - Baladi Black (0.87) followed by Gabali - Baladi Red (0.86), Gabali, Baladi Black (0.82), New Zealand White - Gabali (0.80), New Zealand White - Baladi Red (0.75) and the lowest genetic distance was found between New Zealand White - Baladi Black(0.73). One primer (OPA-20) in Gabali, two primers (OPA-02, OPB-14) in Baladi Red, three primers (OPA-02, OPB-14, OPA-20) in New Zealand White and Baladi Black were found to be specific for these breeds. The study suggests that RAPD can be successfully utilized for detecting genetic variation among the studied rabbit breeds.