الفهرس 
AcknowledgmentOnly 14 pages are availabe for public view
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Abstract
Brucellosis is a zoon tic disease that exist worldwide especially in the Mediterranean basin and it ranks after salmonellosis and tuberculosis as the most important systemic infection causing fever of unknown etiology. Several studies were conducted in different government e.g. Menofeia, Dakahlia, Fayoum, Banha, etc………. To estimate the different methods for diagnosis of brucellosis. The present study was designed to evaluate the rapid and recent diagnosis for brucellosis and to differentiate between the different species which will help in rapid treatment and good prevention of brucellosis. The study sample, which included 81 subjects, the persons chosen among different occupation (farmers, slaughter house workers, veterinarians) from different places in Assuit province in the period from October 2010 until February 2012.As a control groups, 30 apparently healthy persons. All persons subjected to the following: Through history taking searching for history of fever, night sweating, bone pain, spine pain, arthralgia, clinical examination for suggestive manifestation of brucellosis e.g lymphadeneopathy, splenomegaly, hepatomegally, abdominal ultrasound, laboratory investigation (Complete blood count , Liver function tests, Malta agglutination test, Enzyme Linked Immunosorbent Assay (ELISA)for detection of brucella antibody IgM/IgG, Polymeraise Chain Reaction (PCR)for Brucella spp. In our study, the mean age groups range from (18-70years old) with mean±SD(41.69±12.01), as regarding the sex the male was(62)with percentage (76.5%), the female number was(19)with percentage(23.5%).As regard the symptoms & signs of brucella includes fever, history of contact with animals, sweating, bone pain, spine pain, arthlagia, lympadenopathy, there was statistically significant difference between both studied group as regard contact with animals, bone pain, and arthralgia, while no statistically significant difference between the two studied group as regard sex, age, history of fever, spine pain, lymphadenopathy. The majority of this group found in butcher with (37%) followed by housewife (23.5%), then both farmers and veterinarian (12.3%), lastly officers(9.9%). There was no statistically significant difference between both studied groups regarding complete blood count and liver function tests except for hemoglobin concentration median range (14.7g/dl ) (11.7-22.1g/dl) with p value =0.004. Also, there was no statistically significant difference between the studied groups as regard abdominal ultrasound. In our study, as regard ELISA (IgM/IgG) antibodies for detection of Brucella shown that IgM predominance with 11(13.6%) cases are positive from total( 81) cases in group 1 and 2(6.7%) cases positive from group2 ( total 30 persons) whileIgG results negative in both groups. The percentage of the studied groups according to Malta titer, the percentage in less than 1/80 was(21%)in group 1and(83.3%)in group 2; at1/80 percentage was (21%) in group1 and (16.7%)in group2; at 1/60 percentage was (21%) in group1 and (0%) in group2; at 1/320 percentage was (22.2%) in group1 and (0%) in group2; at 1/640percentage was (14.8%) in group1 and (0%) in group 2. The percentage of positive PCR(8) cases represented percentage (81.8%);while the Brucella positive IgM (11)cases represent (13.6%). B.abortus. represents all cases positive by PCR test. The sensitivity of ELISA (IgM)100%, and the specificity (97.22%) and the accuracy(97.53%), sensitivity of PCR(81.82%), specificity (100%), the accuracy (97.5%).