الفهرس | Only 14 pages are availabe for public view |
Abstract The goal of this work is to study the defensin genes in healthy and infected rabbits from V-line Alexandria line. Histological sections and hematological test were done, also the differences of the beta-defensin gene using specific primers and PCR technique, in addition to the sequencing were performed in all healthy mothers (control) and infected rabbits from both lines. The resulting sequences were compared with those in GenBank, and similarities between samples were examined using the program of (EMBOSS Needle) (http://www.ebi.ac.uk/Tools/psa/emboss_needle/nucleotide.html). The phylogenic trees that illustrated similarities and differences between the beta-defensin genes were performed using the MEGA6 program.The differences between serum proteins, using the SDS-PAGE technique, were tested in studied samples.The samples were taken from 30 healthy and infected rabbits from two lines Alexandria and V-line which were divided into groups depending on the health and age. |