الفهرس 
OVARIAN CANCEROnly 14 pages are availabe for public view
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Abstract
ABSTRACT
Minichromosomal Maintenance protein 7 (MCM7), a member of the MCM family of proteins critical to the DNA replication complex, has recently been shown to improve the sensitivity and specificity of Epithelial Ovarian Cancer (EOC) diagnosis but its function in cancer cells is not clear. We evaluated MCM7 expression, RMI and CA125 serum level as diagnostic tools of primary ovarian cancer in Egyptian women. The MCM7 gene expression was evaluated by real time PCR in ovarian cancer of 50 Egyptian women. Ovarian cancer tissues were studied for the detection of the gene expression of MCM7 by Quantitative Real Time PCR (Q RT-PCR). Serum Human cancer antigen 125 (CA 125) was measured in the serum of all participants of the study using immune sorbent assay (ELISA). The MCM7 showed significant difference among ovarian malignant tumors patients compared to the control subjects (p< 0.01). The best cutoff value 1.96 at which MCM7 sensitivity was 100% and specificity was 96%. There was a significance correlation between MCM7, RMI and CA125 in all patients of the study (p#0.01 for both). The mRNA expression of MCM7 was significantly high versus the control group in early stages and low grades of the disease (p = 0.00, 0.01, respectively). As well as, there was Increased MCM7 expression in the late stages of the disease suggesting that it may be associated with poor prognosis as well. The MCM7 could be considered as a good prognostic marker for ovarian cancer that increases the sensitivity of the CA 125 to absolute value without affection of CA125 accuracy and its positive predictive value.
Key words: Ovarian Cancer, RMI, MCM7, real time PCR, CA 125