الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Studies were made on 39 different microbial cultures (15 fungi and 24 bacteria), Ps. fluorescens was found to be the most active organism.
The highest prednisolone yield (63.4 %) was obtained by using 5 mg of cortisoV50 ml medium and addition of0.6 rnVl of H202 at optimum conditions, transformation medium (beef extract,
3; peptone, 5 and glucose, 10 g/1), pH 6.5, temperature 30°C,
culture age 48 hand agitation speed 200 rpm.
Ca-alginate, polyacrylamide and poly-HEMA were used to transfonn cortisol. Poly-HEMA immobilized cells was chosen, the prednisolone yield was increased to 84.3 %by the repeated use of the immobilized cells for 7 cycles. Prednisolone yield reached to its maximum (96.8 . %) by the repeated use of the immobilized cells for 9 cycles by using 2L fennentor.
Isolation and identification of the transfonnation products were carried out by using modem teclmiques.