الفهرس 
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Abstract
RHD is caused by a positive-sense, single-stranded RNA virus, a member of the genus Lagovirus within the family Caliciviridae
Specific antisera to individual immunoglobulin classes are valuable reagents in many immunological techniques such as indirect immune fluorescent technique. Specific, rapid and sensitive serological tests like fluorescent antibody technique (FAT) are required for accurate diagnosis of infectious agents to reach to well applicable control measures.
The present work aims to prepare and compare between different specific anti-RHDV sera prepared in different hosts (rabbits; Guinea pigs and goats) free and conjugated with flourescin isothiocyanate in order to be used for detection and diagnosis of RHDV.
The present obtained results revealed that:
1-Estimation of serum proteins in the prepared anti-RHD sera showed that rabbit serum had the levels of 5.45±0.11;1.85±0.22 and 3.60±0.12g/dl for total serum; albumin and globulin respectively; goat serum had the values of 5.82±0.21; 1.79±0.32 and 4.03±0.10g/dlfor total serum; albumin and globulin respectively while Guinea pig serum had the levels of 5.10±0.13; 1.80±0.15 and 3.2±0.10g/dl for total serum; albumin and globulin respectively.
2-Rabbit; Guinea pigs and goat anti-RHDV hyper immune sera were found to be free from foreign contaminants (aerobic and anaerobic bacteria; fungi and mycoplasma). These sera were titrated using HI test which revealed gradual increasing in RHDV antibodies recorded their peaks by the 5th week post animal immunization as 8; 7 and 8.5log2/ml in rabbit; Guinea pig and goat’s sera respectively showing that goat serum had the highest levels of RHDV antibodies.
3-All experimentally infected rabbits with RHDV showed typical characteristic signs of RHD within three days post infection. Such signs were represented by respiratory distress, bloody nasal discharges; diarrhea and death.
4-The post mortem examination of dead rabbits showed the presence of punctuates hemorrhages in the respiratory system, liver, spleen, cardiac muscle and occasionally in kidney.
5-RHD virus was determined in lung and liver homogenates from died rabbits through application of HI test and direct FAT to confirm that the observed signs were due to RHDV.
6-Application of direct fluorescent antibody technique on lung and liver smears prepared from experimentally infected rabbits using 10 fold dilutions of the prepared conjugated antisera with FITC; revealed that rabbit; Guinea pig and goat sera showed clear strong positive reactions (3-4+) as apple green dots up to dilution of 1:100; 1:100 and 1:1000 respectively. Moderate positive FAT (2+) was determined using 1:10000 dilution of rabbit serum while such degree was obtained with a dilution of 1:100000 using goat serum. Weak reaction (+) was noticed with a dilution of 1:100000 of rabbit serum and 1:10000 of Guinea pig serum and 1:1000000 of goat serum. Further dilutions reveal.