الفهرس 
Chapter 1Only 14 pages are availabe for public view
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Abstract
The biotechnological potential of pectinolytic enzymes from microorganisms has drawn a great deal of attention from various researchers worldwide as likely biological catalysts in a variety of industrial processes. Alkaline pectinases are among the most important industrial enzymes and are of great significance in the current biotechnological area with wide-ranging applications in textile processing, degumming of plant bast fibers, treatment of pectic wastewaters, paper making, and coffee and tea fermentations. The present study highlights the production of polygalacturonase (PGase) form microorganism using low cost substrate for the production. This can be an alternative way to turn this waste into a value-added product, contributing to the reduction of environmental waste disposal problems. Then purification of the enzyme was followed by studying some of its characteristics.
The thesis concerning the present study is dealing with three chapters:
The first chapter is the introduction, in which a survey on polygalacturonases (PGase)s highlighting their substrates, sources, production, mechanism of action, optimization and purification as well as their applications.
The second chapter involved Materials and Methods, in this context;
1- Screening program aimed to select a promising PGase producer bacterial strain from 16 bacterial isolates included in this study (12 isolates were isolated in the present study and 4 isolates were as a gift from Institute of Graduate Studies and Research), qualitatively through tooth-picking technique and quanitatively through estimation the reducing sugars produced by PGase activity using 3,5-dinitrosalycilic acid (DNS) method.
2- Optimization of PGase production from the most potent producer through three steps plan via Submerged Fermentation (SmF)., included.