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Abstract options (Lin, 2008). Considerable morbidity from long-term dialysis treatment and the ever increasing organ transplant waiting lists, indicated clearly a need for new renal replacement therapies (Ricardo and Deane, 2005). In the last few years, reports of bone marrow cell conversion into various types of renal cells have emerged. MSCs have also been shown to provide renal protection by paracrine effects. The diverse developmental potential of bone marrow stem cells as well as the easy access to HSCs and expandable nature of MSCs in cultures opens the possibility of stem cell-based therapy for kidney diseases (Lin, 2008). The aim of the work was to produce renal cells or tissue from MSCs that were isolated from WJ of the UCs hoping to be used in future in treatment of renal diseases. In the present study, 6 UCs from CS deliveries of healthy pregnant mothers were processed and cultured in special medium for 4 weeks with continuous changing of the medium once per week, for isolation and expansion of MSCs from WJ of the UCs. MSCs were further cultured in special 96 well-plate coated with polyamine. Five different growth factors cocktails (protocols) were added for induction of differentiation of MSCs into renal stem cells. Immunophenotype characterization for identification of MSCs surface marker (CD29) and renal progenitor stem cells surface markers (CD24 & CD133) were done by ICC and flowcytometric analysis using monoclonal antibodies anti-CD29, CD24 and CD133, before and after induction of differentiation. |