الفهرس 
Acne VulgarisOnly 14 pages are availabe for public view
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Abstract
Acne is the most common skin disease affecting the vast majority of adolescents and young adults. It is one of the commonest dermatological problems encountered in the out-patient clinics of dermatology.
Severe scarring is a serious complication of acne which associated with considerable psychological distress, poor self-esteem, depression, anxiety, body image alterations, embarrassment, anger, low academic performance, and even unemployment.
Platelet rich plasma is an autologous platelet concentrate which means ”abundant platelets that are concentrated into a small volume of plasma. PRP is obtained by centrifugation of patient blood in a two-steps centri¬fuging process. The initial centrifugation is low speed centrifugation which sepa¬rate platelet poor plasma (PPP) from red blood cells (RBCs) and then followed by high speed one, resulting in two parts as well; upper PPP and lower PRP. Then PRP is activated by calcium chloride to stimulate growth factors secretion and beginning of tissue regeneration cascade.
PRP contain abundant GFs such as PDGF, TGF, EGF, IGF- 1, FGF, HGF, ECGF and VEGF. These growth factors contribute to the acceleration of tissue regeneration and collagen synthesis. Such properties of PRP make it a potential treatment modality for tissue regeneration.
This study aimed to evaluate the safety and efficacy of PRP intradermal injection in treatment of atrophic post acne scars.
This study was carried out on 20 patients complaining of atrophic post acne scars. They were selected from out-patient clinic of dermatology and venereology department of Eldemerdash Hospital Ain Shams University.
All the patients were subjected to complete history taking, general examination and dermatological examination, Routine laboratory investigations, Evaluation of acne scars, their types icepicks, boxcar or rolling, and their grades either mild, moderate or severe were identified. Homogeneity and texture of the skin around was also evaluated, digital photographs were taken at baseline, before each session, one week after last session and during follow up period (2 months after the last session), Punch biopsy (3mm) were taken from atrophic post acne scars from patient face before and one week after 4th session of PRP injection for Histopathological and Immunohistochemistry for collagen III,PRP was prepared by two steps centrifugation technique, then Intradermal injection of PRP in acne scars was done every 2 weeks for 4 sessions followed by follow up period of 2 months and at last written consent was taken from every patient.
Assessment of the efficacy of the procedure by Clinical assessment which occurs by Photographs was taken at baseline, before each session and in the follow up period (2 months), two dermatologists were asked to record percentage of improvement for each patient regarding severity grade, scar type, comparing before and after digital photographs, and the degree of improvement in texture and homogeneity of facial skin was assessed as it became much worse, worse, slightly improved, improved, improved greatly.
Histopathological and immunohistochemistry of collagen III evaluation revealed that increase in collagen amount and quality (more healthy) after 4 sessions of PRP injection, and the patient satisfaction was evaluated using the following grades: slightly satisfied, satisfied, and Very satisfied.
Safety assessments of the patients were informed to report any complications occurred; erythema, pain, ecchymosis, infection or any allergic reactions, etc….
Follow up assessment for 2 months after the last session to detect any improvement or worsening of acne scars and to detect any complications.
Results recorded by clinical assessment in this study, 90% of patients were satisfied to very satisfied, 10% were slightly satisfied, and no patient was dissatisfied.
According to the degree of improvement of each scar grade, (40%) of patients show marked to excellent improvement, (45%) of patients show moderate improvement, and (15%) show mild improvement.
Safety assessment done by reported side effects were few and minor in all patients. Pain during injection was tolerable. Erythema was mild in most patients.
Lastly, follow up assessment report that there was neither further improvement nor worsening of acne scars as there were no differences are observed between scar grades after treatment and after follow up period.