الفهرس 
General IntroductionOnly 14 pages are availabe for public view
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Abstract
The work presented in the current thesis represents plant tissue culture of two medicinal aromatic plants (Foeniculum vulgare Mill. and Coriandrum sativum L.) and biological screening of cytotoxicity, antioxidant, and immunostimulant activities of their extracted oils.
The thesis consists of three parts:
Part I
Plant tissue culture of Foeniculum vulgare Mill. and Coriandrum sativum L. and examination of calli for cytodifferentiation and organogenesis.
Since plant tissue culture systems represent a potential renewable source of valuable medicinal compounds which cannot be produced by microbial cells or chemical synthesis. So, we applied this technology on two important aromatic plants which can be widely used for medicinal and non- medicinal purposes all over the world. The formed calli and tissues from culture system indicates the formation of different organs and xylem tissue which refers to a high degree of cytodifferentiation and these tissues were microscopically examined.
Part II
A. Extraction of essential oil from Foeniculum vulgare Mill. and Coriandrum sativum L. calli and assessment of the yield.
B. Gas chromatography-Mass spectral analysis (GC/MS) of the extracted oils.
Since the main purpose of our tissue culture work was the production of secondary metabolites of interest. So, essential oils for both cultures were extracted and their yield of essential oil was obtained at different time intervals of the culture time. Gas chromatography-Mass spectral analysis was essential for identification of components in the extracted oils.
Part III
Chapter 1- Cytotoxcic activity of essential oil of Foeniculum vulgare Mill. and Coriandrum sativum L. on normal peripheral blood cells (PBMCs), hepatocellular carcinoma (HepG-2) and colorectal adenocarcinoma (Caco-2).
Cancer research is a promising area for work. Discovery of new safe cytotoxic agent is paid a great effort. In this thesis, the extracted oils from cultures were tested for their cytotoxicity against both normal peripheral blood cells (PBMCs) to determine the safe concentrations that can be used next in testing cytotoxocity against two different cancerous cell lines; hepatocellular carcinoma (HepG-
2) and colorectal adenocarcinoma (Caco-2). Both of the tested oils showed no toxicity against normal cells and they showed significant anticancer activity against the tested cancer cell lines.
Chapter 2- Antioxidant activity of essential oil of Foeniculum vulgare Mill. and Coriandrum sativum L. using DPPH assay.
Natural products from dietary components such as indian spices and medicinal plants are known to possess antioxidant activity, there are epidemiological evidences correlating higher intake of foods with antioxidant abilities to lower incidence of various human mortalities. We performed, here screening of antioxidant activity for both of the extracted oils, both of them showed antioxidant activity in vitro by reducing absorbance of DPPH radical in a quite low doses.
Chapter 3- Immunostimulant activity of essential oil of Foeniculum vulgare Mill. and Coriandrum sativum L.
Here, we preformed a biological screening for both extracted oils to show if they have a significant immunostimulant activity or not. Only essential oil extracted from calli of Coriandrum sativum showed a significant immunostimulant activity with a stimulation index value more than 1.5 at a certain concentration.