الفهرس 
AbbreviationsOnly 14 pages are availabe for public view
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Abstract
Urinary tract infection is one of the most common bacterial infections and Gram- negative bacteria are among the most prevalent bacteria detected from UTI patients, especialy Escherichia coli..
Uropathogenic Escherichia coli (UPEC) are associated with acute infections as well as chronic and recurrent infections that require long-term antibiotic therapy and are often associated with life threatening sequelae that can include sepsis and shock.
Our study was conducted in Sohag University Hospital in the period between May 2014 to May 2015. 200 urine samples were collected from outpatients (community acquired infection) and inpatients (hospital acquired infection) either catheterized patients or not. 130 of the patients were males and 70 were females with age ranging from 2 to 80 years.
The specimens were Gram stained and cultured on MaCconkey agar,CLED with Andrade indicator agar and EMB agar .Then tested by oxidase test and RAPID ID one test .Then tested for antibiotic sensitivity by two tests: Disk diffusion test and Double disc diffusion test (DDDT)for ESBL detection. And the genes of ESBL resistence was detected by PCR.
Fourty four specimens (22%) were isolated as E.coli, 16(22.2%) of them were outpatients, 20(29%) of them were hospitalized catheterized patients and 8(13.6%) of them were hospitalized noncatheterized patients.39(88.6%) of E.coli were ESBL producing.
The rate of infection among females was higher than in males and higher in hospitalized catheterized patients than outpatients and hospitalized noncatheterized patients.
Nitrofurantoin showed a 100% efficacy against E coli. Impenem, gentamycin, sulbactam-srfoperazone and amikacin showed over 90% efficacy and lastly ertapenem and meropenem and showed over 80% efficacy.
One hundred percent of hospitalized catheterized patients had an ESBL producing E.coli urinary tract infection.
ESBL genes percentages were:CTX-M (86.4%). TEM was positive in nearly half of cases (47.7%) and lastly SHV was only positive in 4 cases (9.1%).
ESBL-producing E. coli showed 100% resistance rates to fluoroquinolones and ciprofloxacin but were sensitive to aminoglycosides and trimethoprim-sulfamethoxazole by 95%.