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العنوان
Enhanced Detection of Methicillin Resistant
Staphylococcus aureus (MRSA) &
Effect of some Local Agents on them
/
المؤلف
Soliman,Amal Mohammed Sayed
هيئة الاعداد
باحث / أمل محمد سيد سليمان
مشرف / ليلي السيد أحمد سليمان
مشرف / /صافية حامد عبد العزيز العبد
تاريخ النشر
2016.
عدد الصفحات
228.p;
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علم المناعة والحساسية
تاريخ الإجازة
1/6/2016
مكان الإجازة
جامعة عين شمس - كلية الطب - Medical Microbiology & Immunology
الفهرس
Only 14 pages are availabe for public view

from 228

from 228

Abstract

In the present study, we use chrOMagar TM medium as a rapid method taking only 24 hrs. for MRSA detection from 70 clinical specimens (exudates or pus) and 25 nasal swabs (health care personnel) in comparison to the conventional method which needs 48 hrs., the validity values obtained were 100% sensitivity, specificity, PPV and NPV of cefoxitin disc diffusion method versus 93.8% sensitivity, 100% specificity, 100% PPV and 97% NPV of the chromogenic agar in identification of MRSA isolates from nasal swabs, burn and wound discharge samples collectively. Also we test the effect of some antiseptics Agents on the MRSA isolates. The used antiseptics were acetic acid (5%), betadine (10%), sodium hypochlorite´´ dakin’s solution´´ (4%) and H2O2 (10%). Minimal inhibitory concentration (MIC) & minimal bactericidal concentration (MBC) of the different antiseptics were measured against the MRSA isolates. These results revealed that acetic acid was effective in the range of 5% to 0.63%, while betadine was effective in the range of 10% to 1.25%, also H2O2 was effective in the range of 10% to 0.63% but Dakin’s solution was effective in the range of 0.5% to 0.063%.