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العنوان
Typing of Clostridium perfringens isolated from necrotic enteritis in turkeys by multiplex PCR /
المؤلف
Ashour, Amany Elsayed Mahmoud.
هيئة الاعداد
باحث / امانى السيد محمود عاشور
مشرف / هايدى محمد شوقى
مشرف / محمد كمال رفاعى
الموضوع
Clostridium perfringens.
تاريخ النشر
2016.
عدد الصفحات
130 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
1/1/2016
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Microbiology
الفهرس
Only 14 pages are availabe for public view

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Abstract

A total of 122 intestinal samples were collected from diseased (suffering from necrotic enteritis (NE)) or apparently healthy turkey poults at different ages (2 months to 5 months age old) and seasons (summer & winter). Most of the samples were obtained from turkey poult flocks in Kafr Ghataty’s farm, Giza, Governorate. The bacteriological examination revealed that 56 (45.9%) of the samples were culture positive. Clostridium perfringens was confirmed by biochemical characters and was analyzed by multiplex PCR in order to determine the toxin genes for the molecular typing. Type A without enterotoxin was recovered from 53 isolates (43.4%). One isolate was type A with enterotoxins (0.82%), another isolate was Type C (0.82%), and a third isolate was type E (0.82%). Types B and D were not identified.