الفهرس 
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Abstract
Rate of isolation of CPS and CNS were 15% and zero % in clinical mastitic farm cows, respectively. Incidence of CPS and CNS were 20.3% and 12.2 % in clinical mastitic individual cows, respectively. Incidence of CPS and CNS were 36.8% and 22.8 % in clinical mastitic buffaloes, respectively. Briefly, incidence of clinical CPS mastitis most common among our study population than clinical CNS mastitis. Rate of isolation of CPS and CNS in were 30% and 20 % in sub clinical mastitic farm cows, respectively. Incidence of CPS and CNS were 66.6% and 5.5 % in sub clinical mastitic individual cows, respectively. Incidence of CPS and CNS were 33.3% and zero % in sub clinical mastitic buffaloes, respectively. Briefly, incidence of subclinical CPS mastitis most common among our study population than sub clinical CNS mastitis. Clinical signs of CPS and CNS in mastitis vary from per acute, acute and chronic among bovine population. Incidence of per acute mastitis occurs in 31 cases (11 CPS and 20 CNS). Incidence of acute mastitis occurs in 61 cases (47 CPS and 14 CNS). Incidence of chronic mastitis occurs in 20 cases (14 CPS and 6 CNS). Briefly, acute staphylococcal mastitis was the most common form of mastitis in this study. Individual cows were higher affected by per acute CPS mastitis (17.7%) while buffaloes were higher affected by acute CPS mastitis (71.4%) while farm cows were higher affected by chronic CPS mastitis (33.3%). Buffaloes were higher affected by per acute and acute CNS mastitis (53.8% and 38.4% respectively) while Individual cows were higher affected by chronic CNS mastitis (18.5%). Seasonal occurrence of mastitis occurs in descending matter 41.4%, 37.1%, 15.2% and 5.6% in spring, winter, autumn and summer, respectively. Winter season was the most common season for incidence of CPS mastitis in farm cows (50%). While spring season was the most common season for incidence of CPS mastitis in individual cows and buffaloes (47.6% and 79.1% respectively). Winter season was the most common season for incidence of CNS mastitis in farm cows (100%). While spring season was the most common season for incidence of CNS mastitis in individual cows and buffaloes (64.2% and 53.8% respectively). Rate of isolation of staphylococci in mastitis according to stage of lactation occurs in descending matter 56.9%, 34.5% and 8.5% in 3-6 month, 1-2 month and 7-9 month post calving, respectively. 3-6 month post calving was most common months for incidence of CPS in farm cows and buffaloes (75% and 54.1% respectively) while 1-2 month post calving was most common months for incidence of CPS in individual cows (66.6%). 3-6 month post calving was most common months for incidence of CNS in farm cows and individual cows (100% and 46.4% respectively) while 1-2 month post calving was most common months for incidence of CNS in buffaloes (69.2%). occurrence of staphylococcal mastitis according to age of animal occurs in descending matter 37.9%, 17.3%, 17.1%, 17.1% and 10.4% in 3-4 years old, 5-6 years old, 6-7 years old, 7 years old and 4-5 years old. 4-5 years old was most common for incidence of CPS in farm cows and individual cows (58.3% and 42.8% respectively) while 7 years old was most common for incidence of CPS in buffaloes (54.1%). 4-5 years old was most common for incidence of CNS in farm cows (75%). 7 years old was most common for incidence of CNS in individual cows (60.7). 6-7 years old was most common for incidence of CNS in buffaloes (60.5%). 51 CPS isolates more resistant to Amoxicillin plus clauvilinic acid, Trimeth/sulfa, Tetracycline, Ampicillin plus sulbectam and Penicillin G in descending matter. CPS more sensitive to Enrofloxacin, Marbofloxacine, Florofenicole, Cefaquinom and Gentamycin in descending matter. 10 CNS isolates more resistant to Tetracycline, Lincomycin plus spectinomycin, Cefotaxime, Ampicillin plus sulbectam, Amoxicillin and clauvilinic and Trimeth/sulfa in descending matter. CNS more sensitive to Enrofloxacin, Marbofloxacine, Cefaquinom, and Florofenicol in descending matter. Agarose gel electrophoresis of the amplified PCR fragments for the staphylococcal toxin genes Sea, sec, see and tst on 19 samples. Lane 1,2,3,4,5,6,7,8,9,11,12,13,14,15 positive for SEC toxin. Lane 10,16,17,18,19 negative for SEC toxin. All samples negative for sea, see and tst toxin.