الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 111 |  |  |
| | | from | 111 | | |
Abstract
In this dissertation, we study the incidence of tubercle bacilli in milk and milk products (kareish cheese and fresh cream).Two hundreds and fourty two samples (100 milk ,65 kareish cheese and 77 fresh cream) were collected randomly from dairy shops, markets and farmer’s animals at different localities in Beni-Suef city.
These samples were cultured, microscopically examined and confirmed by rapid methods (real time-PCR) and the results revealed that the incidence of tuberculosis was 12 positivesamples forMycobacterium boviswith a percentage of 4.96% by conventional method and 17 samples were positives with a percentage 7.02% by PCR.
Regarding to conventional culture technique, 6 out of 100milk samples were positive to Mycobacterium bovis with a percentage 6% , while 2 out of 77 cream sampleswere positive with a percentage 2.59% and 4 out of 65 kareish cheese samples were positive with a percentage 6.15%.
By using Polymerase Chain Reaction method, the obtained results were 8 out of 100 raw milk samples positive with a percentage of 8%, while in other samples 6 out of 65 kareish cheese samples were positivewith a percentage of 9.23% and 3 out of 77 fresh cream sampleswere positivewith a percentage of 3.89%, all tested isolates of mycobacterium spp. were confirmed as Mycobacterium bovis with percentage (100%) by using the primers of Mycobacterium bovis.
Real time PCR is more accurate and faster than conventional method for TB diagnosis; as it’s measured DNA of all samples either live or dead mycobacteria,while culturing method isolates only viable mycobacteria ,so we found that the results were higher in PCR than conventional method.